DNMT3B/3L Methyltransferase Assay Service

Activity-based DNMT3B/3L Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
DNMT3B/3L
Enzyme
Co-expressed Human DNMT3B/3L catalytic domain
Synonym
DNMT3B: DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB, DNM3B; DNMT3L: DNA (cytosine-5)-methyltransferase 3-like, DNM3L
Substrates
Lambda DNA, 0.0075 mg/mlSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + DNA = S-adenosyl-L-homocysteine + DNA 5-[methyl-³H]-cytosine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH146
Sinefungin210
Scientific Information
DNMT3B: Q9UBC3 (http://www.uniprot.org/uniprot/Q9UBC3)DNMT3L: Q9UJW3 (http://www.uniprot.org/uniprot/Q9UJW3)
Screening Location
Malvern, USA
Reference compound IC50 for DNMT3B/3L
Reference compound IC50 for DNMT3B/3L