DNMT3B Methyltransferase Assay Service

Activity-based DNMT3B Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
DNMT3B
Enzyme
Human DNMT3B-full length
Synonym
DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB, DNM3B
Substrates
Lambda DNA, 0.0075 mg/mlSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + DNA = S-adenosyl-L-homocysteine + DNA 5-[methyl-³H]-cytosine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH122
Sinefungin140
Scientific Information
Q9UBC3 (http://www.uniprot.org/uniprot/Q9UBC3)
Screening Location
Malvern, USA
Reference compound IC50 for DNMT3B
Reference compound IC50 for DNMT3B