DOP Functional Cell-based Agonist Assay for compound screening and profiling via fluorescence-based quantification of intracellular calcium.
Target Overview
CAT#
DOP Functional Agonist
GPCR Target
Human DOP
Cell Line
CHO-K1/OPRD1/Gα15
Scientific Information
OPRD1 (https://www.uniprot.org/uniprotkb/P41143/entry)
Assay Properties
Protocol
DOP activation by agonist application using FLIPR Penta system cells treated
with Calcium 6 dye; fluorescence kinetic measurement
Readout
Calcium 6 dye fluorescence tracking changes in intracellular calcium
Reference Compound EC50
| Compounds | EC50 (nM) |
|---|---|
| DADLE | 3.6 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website
Reference compound activation of DOP
PDF