DOP Functional Cell-based Antagonist Assay for compound screening and profiling via fluorescence-based quantification of intracellular calcium.
Target Overview
CAT#
DOP Functional Antagonist
GPCR Target
Human DOP
Cell Line
CHO-K1/OPRD1/Gα15
Scientific Information
OPRD1 (https://www.uniprot.org/uniprotkb/P41143/entry)
Assay Properties
Protocol
30 min incubation with antagonist; DOP activation by agonist application
using FLIPR Penta system cells treated with Calcium 6 dye; fluorescence
kinetic measurement
Readout
Calcium 6 dye fluorescence tracking changes in intracellular calcium
Reference Compound EC50
| Compounds | IC50 (nM) |
|---|---|
| Naltrexone | >30000 |
| Naltrindole | 8.9 |
| Aticaprant | >10000 |
Agonist = 30 nM DADLE
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website
Reference compound inhibition of DOP
PDF