Activity-based DOT1L Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Histone-lysine N-methyltransferase, H3 lysine-79 specific, Histone H3-K79 methyltransferase, H3-K79-HMTase, DOT1-like protein, Lysine N-methyltransferase 4, KIAA1814, KMT4
Oligo Nucleosomes, 0.05 mg/ml
SAM, 1 µM
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Malvern, PA, USA
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for DOT1