Activity-based DYRK1B kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
DYRK1B
Enzyme
Human DYRK1B
HGNC
DYRK1B
Synonym
MIRK
Substrates
Peptide substrate, [RRRFRPASPLRGPPK], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.0 |
| Ro 31-8220 | 68 |
| GW 5074 | 470 |
| H-89 | >20,000 |
| Rottlerin | >20,000 |
Scientific Information
Q9Y463 (http://www.uniprot.org/uniprot/Q9Y463)
Screening Location
Malvern, USA
Reference compound IC50 for DYRK1B
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