DYRK3 Kinase Assay Service

Activity-based DYRK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
DYRK3

Enzyme
Human DYRK3

HGNC
DYRK3

Synonym
DYRK5, RED, REDK

Substrates
Peptide substrate, [RRRFRPASPLRGPPK], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 29
Ro 31-8220 580
GW 5074 540
H-89 7,000
Rottlerin >20,000
Scientific Information
O43781 (http://www.uniprot.org/uniprot/O43781)

Screening Location
Malvern, USA

Reference compound IC50 for DYRK3
Reference compound IC50 for DYRK3