DYRK3 Kinase Assay Service

Activity-based DYRK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
DYRK3
Enzyme
Human DYRK3
HGNC
DYRK3
Synonym
DYRK5, RED, REDK
Substrates
Peptide substrate, [RRRFRPASPLRGPPK], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine29
Ro 31-8220580
GW 5074540
H-897,000
Rottlerin>20,000
Scientific Information
O43781 (http://www.uniprot.org/uniprot/O43781)
Screening Location
Malvern, USA
Reference compound IC50 for DYRK3
Reference compound IC50 for DYRK3