DYRK4 Kinase Assay Service

Activity-based DYRK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
DYRK4

Enzyme
Human DYRK4

HGNC
DYRK4

Synonym
DYRK4

Substrates
Peptide substrate, [RRRFRPASPLRGPPK], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 18,500
Ro 31-8220 >20,000
GW5074 11,500
H-89 >20,000
Rottlerin >20,000
Scientific Information
Q9NR20 (http://www.uniprot.org/uniprot/Q9NR20)

Screening Location
Malvern, USA

Reference compound IC50 for DYRK4
Reference compound IC50 for DYRK4