Activity-based EEF2K kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
EEF2K
Enzyme
Human EEF2K, wt
HGNC
EEF2K
Manning ID
eEF2K
Synonym
HSU93850, MGC45041, eEF-2K, EEF2K
UniProt
O00418 (EF2K_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Atypical
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-GSK3(14-27), ATP
Reaction
RBER-GSK3(14-27) + 33P-ATP = 33P-RBER-GSK3(14-27) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| NH125 | 6400 |
| Sorafenib | 10000 |
| Sunitinb | 93000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | EEF2K |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
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