EGFR C797S/L858R Kinase Activity Assay Service

Activity-based EGFR C797S/L858R kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

EGFR C797S/L858R

Enzyme

Human EGFR C797S/L858R, mutant

HGNC

EGFR (C797S/L858R)

Manning ID

EGFR

Synonym

EGFR, ERBB, ERBB1, mENA, EGF-R C797S/L858R, EGFR C797S/L858R, EGFR (C797S/L858R)

UniProt

P00533 (EGFR_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

PolyEY, ATP

Reaction

PolyEY + 33P-ATP = 33P-PolyEY + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 110
Sorafenib 170000
Sunitinb 100000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice EGFR C797S/L858R
345 wt Profiler No
345 IC50 wt Profiler No
Mutant Profiler Addon Yes
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – EGFR C797S/L858R
(Activity verified by 33PanQinaseTM-Assay)

Product Number 1709-0000-1
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