EGFR (d746-750/C797S) Kinase Assay Service

Activity-based EGFR (d746-750/C797S) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
EGFR (d746-750/C797S)
Enzyme
Human EGFR (d746-750/C797S)
HGNC
EGFR (d746-750/C797S)
Synonym
ERBB, mENA, ERBB1
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine14
PP237
AG1478<1.0
PDGFR I-III22,800
D-644063,000
Scientific Information
P00533 (http://www.uniprot.org/uniprot/P00533)
Screening Location
Malvern, USA
Reference compound IC50 for EGFR (d746-750/C797S)
Reference compound IC50 for EGFR (d746-750/C797S)