EGFR (d746-750/G724S) Kinase Assay Service

Activity-based EGFR (d746-750/G724S) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

EGFR (d746-750/G724S)

Enzyme

Human EGFR (d746-750/G724S)

HGNC

EGFR (d746-750/G724S)

Synonym

ERBB, mENA, ERBB1

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1,500
PP2 4,400
AG1478 6.5
EGFR/ErbB Inhibitor 6.4
D-64406 >100,000
Scientific Information

P00533 (http://www.uniprot.org/uniprot/P00533)

Screening Location

Malvern, USA

Reference compound IC50 for EGFR (d746-750/G724S)