EGFR (d746-750/S768I) Kinase Assay Service

Activity-based EGFR (d746-750/S768I) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

EGFR (d746-750/S768I)

Enzyme

Human EGFR (d746-750/S768I)

HGNC

EGFR (d746-750/S768I)

Synonym

ERBB, mENA, ERBB1

Scientific Information

P00533 (http://www.uniprot.org/uniprot/P00533)

Assay Properties

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 21
PP2 76
AG1478 1.9
EGFR/ErbB Inhibitor 2.0
D-64406 7,300

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for EGFR (d746-750/S768I)

Reference compound IC50 for EGFR (d746-750/S768I)