EGFR (d746-750/T790M/C797S/L858R) Kinase Assay Service

Activity-based EGFR (d746-750/T790M/C797S/L858R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#

EGFR (d746-750/T790M/C797S/L858R)

Enzyme

Human EGFR (d746-750/ T790M/C797S/L858R)

HGNC

EGFR (d746-750/T790M/C797S/L858R)

Synonym

Epidermal growth factor receptor, Receptor tyrosine-protein kinase ErbB-1, HER1, EGF-R, ERBB, mENA, ERBB1

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.8
PP2 174
AG1478 350
EGFR/ErbB Inhibitor >100,000
D-64406 4,800
Scientific Information

P00533 (http://www.uniprot.org/uniprot/P00533)

Screening Location

Malvern, USA

Reference compound IC50 for EGFR (d746-750/T790M/C797S/L858R)

Reference compound IC50 for EGFR (d746-750/T790M/C797S/L858R)