EGFR d747-749/A750P Kinase Activity Assay Service

Activity-based EGFR d747-749/A750P kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

EGFR d747-749/A750P

Enzyme

Human EGFR d747-749/A750P, mutant

HGNC

EGFR (d747-749/A750P)

Manning ID

EGFR

Synonym

EGFR, ERBB, mENA, ERBB1, EGF-R d747-749/A750P, EGFR d747-749/A750P, EGFR (d747-749/A750P)

UniProt

P00533 (EGFR_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

PolyEY, ATP

Reaction

PolyEY + 33P-ATP = 33P-PolyEY + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 270
Sorafenib 10000
Sunitinb 42000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice EGFR d747-749/A750P
345 wt Profiler No
345 IC50 wt Profiler No
Mutant Profiler Addon Yes
Lipid Kinase Profiler Addon No
IC50 Profiler IC50 EGFR Profiler
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – EGFR d747-749/A750P
(Activity verified by 33PanQinaseTM-Assay)

Product Number 1038-0000-1
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