EGFR (d747-749/A750P) Kinase Assay Service

Activity-based EGFR (d747-749/A750P) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
EGFR (d747-749/A750P)
Enzyme
Human EGFR (d747-749/A750P)
HGNC
EGFR (d747-749/A750P)
Synonym
ERBB, mENA, ERBB1
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine6.0
PP263
AG1478<0.1
PDGFR I-III20,000
D-644065,400
Scientific Information
P00533 (http://www.uniprot.org/uniprot/P00533)
Screening Location
Malvern, USA
Reference compound IC50 for EGFR (d747-749/A750P)
Reference compound IC50 for EGFR (d747-749/A750P)