EGFR (K716A/C797S/L858R) Kinase Assay Service

Activity-based EGFR (K716A/C797S/L858R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
EGFR (K716A/C797S/L858R)
Enzyme
Human EGFR (K716A/C797S/L858R)
HGNC
EGFR (K716A/C797S/L858R)
Synonym
ERBB, mENA, ERBB1
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine6.1
PP23,100
AG14781,800
EGFR/ErbB Inhibitor>100,000
D-6440658,000
Scientific Information
P00533 (http://www.uniprot.org/uniprot/P00533)
Screening Location
Malvern, USA
Reference compound IC50 for EGFR (K716A/C797S/L858R)
Reference compound IC50 for EGFR (K716A/C797S/L858R)