EGFR (T790M/C797G/L858R) Kinase Assay Service

Activity-based EGFR (T790M/C797G/L858R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

EGFR (T790M/C797G/L858R)

Enzyme

Human EGFR (T790M/C797G/L858R)

HGNC

EGFR (T790M/C797G/L858R)

Synonym

Epidermal growth factor receptor, Receptor tyrosine-protein kinase ErbB-1, HER1

Scientific Information

P00533 (http://www.uniprot.org/uniprot/P00533)

Assay Properties

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.7
PP2 2,800
AG1478 1,900
EGFR/ErbB Inhibitor >1,000
D-64406 67,000

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for EGFR (T790M/C797G/L858R)

Reference compound IC50 for EGFR (T790M/C797G/L858R)