EIF2AK1/HRI Kinase Assay Service

Activity-based EIF2AK1/HRI kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

EIF2AK1

Enzyme

Human EIF2AK1

HGNC

EIF2AK1

Synonym

Eukaryotic translation initiation factor 2-alpha kinase 1, Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Hemin-sensitive initiation factor 2-alpha kinase, Heme-regulated inhibitor, Heme-controlled repressor, HCR, HRI, KIAA1369, PRO1362, E2AK1

Substrates

EIF2S1, 10 µM
ATP, 10 µM

Assay Properties

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 2,650
Ro 31-8220 7,700
GW 5074 16,400
H-89 >100,000
GSK-2606414 310

Scientific Information

Q9BQI3 (http://www.uniprot.org/uniprot/Q9BQI3)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for EIF2AK1

Reference compound IC50 for EIF2AK1/HRI

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