Activity-based EIF2AK2/PKR kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
EIF2AK2
Enzyme
Human EIF2AK2
HGNC
EIF2AK2
Synonym
Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-inducible RNA-dependent protein kinase, Protein kinase RNA-activated, p68 kinase, PKR, PRKR, E2AK2
Substrates
EIF2S1, 10 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 74 |
| Ro 31-8220 | 16,400 |
| GW 5074 | 3,300 |
| H-89 | >100,000 |
| GSK-2606414 | 650 |
Scientific Information
P19525 (http://www.uniprot.org/uniprot/P19525)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for EIF2AK2
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