Activity-based EIF2AK3/PERK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
EIF2AK3
Enzyme
Human EIF2AK3
HGNC
EIF2AK3
Synonym
Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, PEK, E2AK3
Substrates
EIF2S1, 10 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 4,600 |
| Ro 31-8220 | 16,800 |
| GW 5074 | 5,000 |
| H-89 | >100,000 |
| GSK-2606414 | 9.0 |
Scientific Information
Q9NZJ5 (http://www.uniprot.org/uniprot/Q9NZJ5)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for EIF2AK3
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