Activity-based EIF2AK4/GCN2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
EIF2AK4/GCN2
Enzyme
Human EIF2AK4/GCN2
HGNC
EIF2AK4
Synonym
Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein, KIAA1338, E2AK4
Substrates
EIF2S1, 10 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 47 |
| Ro 31-8220 | >100,000 |
| GW 5074 | 11,000 |
| H-89 | >100,000 |
| GSK-2606414 | 2,800 |
Scientific Information
Q9P2K8 (http://www.uniprot.org/uniprot/Q9P2K8)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for EIF2AK4
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