EP300 Bromodomain Assay Service (ThermoShift)

EP300-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview




Human EP300 bromodomain, GST-tagged


Histone acetyltransferase p300, E1A-associated protein p300, p300

Assay Properties


Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (2.4 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)


Reference Compound ΔTms:

SGC-CBP30 – 13 (Red)CBP112 – 9 (Black)BET151 – 6 (Blue)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, RVX-208, GSK2801 and PFI3)

Scientific Information

Q09472 (http://www.uniprot.org/uniprot/Q09472)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services

Melt Curve

Melt Curve

Melt Peak

Melt Peak