EP300 Bromodomain Assay Service (ThermoShift)

EP300-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-EP300-H

Protein

Human EP300 bromodomain, His-tagged

Synonyms

Histone acetyltransferase p300, E1A-associated protein p300, p300

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (5.8 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

40,5

Reference Compound ΔTms:

CBP112 - 6.5 (Black)SGC-CBP30 - 9 (Red)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, RVX-208, GSK2801 and PFI3)

Scientific Information

Q09472 (http://www.uniprot.org/uniprot/Q09472)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services

Melt Curve

Melt Curve

Melt Peak

Melt Peak