Activity-based EPHA2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
EPHA2
Enzyme
Human EPHA2
HGNC
EPHA2
Synonym
ECK
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 53 |
| PP2 | 68 |
| AG1478 | 1,500 |
| D-64406 | 58,000 |
Scientific Information
P29317 (http://www.uniprot.org/uniprot/P29317)
Screening Location
Malvern, USA
Reference compound IC50 for EPHA2
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