EPHA5 Kinase Assay Service

Activity-based EPHA5 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
EPHA5

Enzyme
Human EPHA5

HGNC
EPHA5

Synonym
CEK7, EHK1,HEK7,

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 19
PP2 230
AG1478 4,900
D-64406 >20,000
Scientific Information
P54756 (http://www.uniprot.org/uniprot/P54756)

Screening Location
Malvern, USA

Reference compound IC50 for EPHA5
Reference compound IC50 for EPHA5