EPHB3 Kinase Assay Service

Activity-based EPHB3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
EPHB3
Enzyme
Human EPHB3
HGNC
EPHB3
Synonym
ETK2, HEK2, TYRO6
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine825
PP2107
AG147830,000
Scientific Information
P54753 (http://www.uniprot.org/uniprot/P54753)
Screening Location
Malvern, USA
Reference compound IC50 for EPHB3
Reference compound IC50 for EPHB3