Activity-based EPHB4 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
EPHB4
Enzyme
Human EPHB4, wt
HGNC
EPHB4
Manning ID
EphB4
Synonym
HTK, MYK1, TYRO11, EPHB4
UniProt
P54760 (EPHB4_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2467 |
| Sorafenib | 2700 |
| Sunitinb | 5300 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | EPHB4 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – EPHB4 |
| Product Number | 0178-0000-3 |
| Web Shop | Reaction Biology Online Shop |
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