ERBB2 (A775_G776insYVMA) Kinase Assay Service

Activity-based ERBB2 (A775_G776insYVMA) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#

ERBB2 (A775_G776insYVMA)

Enzyme

Human ERBB2 (A775_G776insYVMA) 

HGNC

ERBB2 (A775_G776insYVMA)

Synonym

NEU, NGL, HER2, TKR1

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 53
PP2 26
AG1478 4.6
EGFR/Erb Inhibitor 0.2
D-64406 7,900

Scientific Information

P04626 (http://www.uniprot.org/uniprot/P04626)

Screening Location

Malvern, USA

Reference compound IC50 for ERBB2 (A775_G776insYVMA)

Reference compound IC50 for ERBB2 (A775_G776insYVMA)