ERBB2 (P1170A) Kinase Assay Service

Activity-based ERBB2 (P1170A) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ERBB2/HER2 (P1170A)
Enzyme
Human ERBB2/HER2 (P1170A)
HGNC
ERBB2 (P1170A)
Synonym
NEU, NGL, HER2, TKR1
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine88
PP2250
AG14789.3
EGFR/Erb Inhibitor0.3
D-64406>100,000
Scientific Information
P04626 (http://www.uniprot.org/uniprot/P04626)
Screening Location
Malvern, USA
Reference compound IC50 for ERBB2/HER2 (P1170A)
Reference compound IC50 for ERBB2/HER2 (P1170A)