Activity-based ERBB2 (P780_Y781INSGSP) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ERBB2 (P780_Y781INSGSP)
Enzyme
Human ERBB2 (P780_Y781INSGSP)
HGNC
ERBB2 (P780_Y781INSGSP)
Synonym
HER2, MLN19, NEU, NGL
Scientific Information
P04626 (http://www.uniprot.org/uniprot/P04626)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 335 |
| Afatinib | 0.2 |
| Gefitinib | 0.9 |
| Lapatinib | 34.8 |
| Dacomitinib | 1.9 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Screening Assay Services.
Reference compound inhibition of ERBB2 (P780_Y781INSGSP)
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