ERK5 Kinase Activity Assay Service

Activity-based ERK5 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview
RBE Target ID

ERK5

Enzyme

Human ERK5, wt

HGNC

MAPK7 (aa5-397)

Manning ID

Erk5

Synonym

BMK1, ERK4, ERK5, PRKM7, BMK1 kinase, MAPK7, ERK5/MAPK7 CD

UniProt

Q13164 (MK07_HUMAN)

Kinase Type

Protein kinase

Kinase Group

CMGC

Assay Properties
Assay Format

33PanQinaseTM

Substrates

RBER-CHKtide, ATP

Reaction

RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 50
Sorafenib 10000
Sunitinib 6000
Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability
Screening Opportunities
FreeChoice ERK5
355 wt Profiler Yes
355 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – ERK5
(Activity verified by 33PanQinaseTM-Assay)
Product Number 1105-0000-1
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