ERK5/MAPK7 FL Kinase Assay Service

Activity-based ERK5/MAPK7 FL kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ERK5/MAPK7
Enzyme
Human ERK5/MAPK7
HGNC
MAPK7
Synonym
BMK1, ERK4, PRKM7
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine10,000
Ro 31-822049,000
GW50749,100
H-89>50,000
Rottlerin>50,000
Scientific Information
Q13164 (http://www.uniprot.org/uniprot/Q13164)
Screening Location
Malvern, USA
Reference compound IC50 for ERK5/MAPK7
Reference compound IC50 for ERK5/MAPK7