ERK7/MAPK15 Kinase Assay Service

Activity-based ERK7/MAPK15 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
ERK7/MAPK15

Enzyme
Human ERK7/MAPK15

HGNC
MAPK15

Synonym
ERK8

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 5.5
Ro 31-8220 0.3
GW5074 1,100
H-89 3,200
Rottlerin 3,500
Scientific Information
Q8TD08 (http://www.uniprot.org/uniprot/Q8TD08)

Screening Location
Malvern, USA

Reference compound IC50 for ERK7/MAPK15
Reference compound IC50 for ERK7/MAPK15

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