ERN1/IRE1 Kinase Assay Service

Activity-based ERN1/IRE1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
ERN1/IRE1

Enzyme
Human ERN1/IRE1

HGNC
ERN1

Synonym
IRE1

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 110
Ro 31-8220 13,500
GW5074 15,000
H-89 >100,000
Rottlerin >100,000
Scientific Information
O75460 (http://www.uniprot.org/uniprot/O75460)

Screening Location
Malvern, USA

Reference compound IC50 for ERN1/IRE1
Reference compound IC50 for ERN1/IRE1

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