ERN1 (R727A) Kinase Assay Service

Activity-based ERN1 (R727A) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#

ERN1/IRE1 (R727A)

Enzyme

Human ERN1/IRE1 (R727A)

HGNC

ERN1 (R727A)

Synonym

Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1

Substrates

MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 37
Ro 31-8220 7,500
GW5074 1,600
H-89 >100,000
Rottlerin >100,000
Scientific Information

O75460 (http://www.uniprot.org/uniprot/O75460)

Screening Location

Malvern, USA

Reference compound IC50 for ERN1/IRE1 (R727A)

Reference compound IC50 for ERN1/IRE1 (R727A)