FAK/PTK2 Kinase Assay Service

Activity-based FAK/PTK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FAK/PTK2

Enzyme
Human FAK/PTK2

HGNC
PTK2

Synonym
FAK, FADK

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 34
Scientific Information
Q05397 (http://www.uniprot.org/uniprot/Q05397)

Screening Location
Malvern, USA

Reference compound IC50 for FAK/PTK2
Reference compound IC50 for FAK/PTK2