Activity-based FGFR1 (W666R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FGFR1 (W666R)
Enzyme
Human FGFR1 (W666R)
HGNC
FGFR1 (W666R)
Synonym
Fibroblast growth factor receptor 1, FGFR-1, bFGF-R, Fms-like tyrosine kinase 2, FLT2, c-fgr
Scientific Information
P11362 (http://www.uniprot.org/uniprot/P11362)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 4.8 |
| PP2 | 490 |
| AG1478 | 10,000 |
| PDGFR I-III | >100,000 |
| D-64406 | 6,300 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for FGFR1 (W666R)
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