FGFR1OP- FGFR1 Kinase Assay Service

Activity-based FGFR1OP- FGFR1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FGFR1OP- FGFR1

Enzyme
Human FGFR1OP- FGFR1 fusion

HGNC
CEP43-FGFR1

Synonym
FOP, CEP43

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 9.2
PP2 1,300
AG1478 8,100
PDGFR I-III >100,000
D-64406 11,000
Scientific Information
FGFR1OP ; O95684 (http://www.uniprot.org/uniprot/O95684)FGFR1 ; P11362 (http://www.uniprot.org/uniprot/P11362)

Screening Location
Malvern, USA

Reference compound IC50 for FGFR1OP-FGFR1
Reference compound IC50 for FGFR1OP-FGFR1

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