FGFR1(V561M) Kinase Assay Service

Activity-based FGFR1(V561M) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FGFR1 (V561M)

Enzyme
Human FGFR1 (V561M)

HGNC
FGFR1 (V561M)

Synonym
H2, H3, H4, H5, CEK, FLG, FLT2, KAL2, BFGFR, C-FGR, N-SAM

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.9
PP2 >100,000
AG1478 >100,000
PDGFR I-III >100,000
D-64406 17,000
Scientific Information
P11362 (http://www.uniprot.org/uniprot/P11362)

Screening Location
Malvern, USA

Reference compound IC50 for FGFR1 (V561M)
Reference compound IC50 for FGFR1 (V561M)