FGFR2 (C491A/V564I) Kinase Assay Service

Activity-based FGFR2 (C491A/V564I) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

FGFR2 (C491A/V564I)

Enzyme

Human FGFR2 (C491A/V564I)

HGNC

FGFR2 (C491A/V564I)

Synonym

Fibroblast growth factor receptor 2 [Precursor], Keratinocyte growth factor receptor 2

Scientific Information

P21802 (http://www.uniprot.org/uniprot/P21802)

Assay Properties

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.0
PP2 >100,000
AG1478 >100,000
EGFR/ErbB Inhibitor >100,000
D-64406 36,000
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for FGFR2 (C491A/V564I)