Activity-based FGFR3 K650M kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
FGFR3 K650M
Enzyme
Human FGFR3 K650M, mutant
HGNC
FGFR3 (K650M)
Manning ID
FGFR3
Synonym
FGFR3, ACH, CD333, CEK2, FGFR-3, HSFGFR3EX, JTK4, FGF-R3 K650M, FGFR3 K650M, FGFR3 (K650M)
UniProt
P22607 (FGFR3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 10 |
| Sorafenib | 2100 |
| Sunitinb | 1200 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | FGFR3 K650M |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – FGFR3 K650M |
| Product Number | 1069-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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