FGFR3 (K650M) Kinase Assay Service

Activity-based FGFR3 (K650M) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FGFR3 (K650M)
Enzyme
Human FGFR3 (K650M)
HGNC
FGFR3 (K650M)
Synonym
ACH, CD333, CEK2, HSFGFR3EX, JTK4
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine20
PP22,000
AG14782,200
PDGFR I-III>50,000
D-6440611,000
Scientific Information
P22607 (http://www.uniprot.org/uniprot/P22607)
Screening Location
Malvern, USA
Reference compound IC50 for FGFR3 (K650M)
Reference compound IC50 for FGFR3 (K650M)