FGFR3 Kinase Assay Service

Activity-based FGFR3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FGFR3
Enzyme
Human FGFR3
HGNC
FGFR3
Synonym
ACH, CD333, CEK2, HSFGFR3EX, JTK4
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine25
PP29,200
AG147822,000
D-644069,000
Scientific Information
P22607 (http://www.uniprot.org/uniprot/P22607)
Screening Location
Malvern, USA
Reference compound IC50 for FGFR3
Reference compound IC50 for FGFR3