FGFR3 Kinase Assay Service

Activity-based FGFR3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FGFR3

Enzyme
Human FGFR3

HGNC
FGFR3

Synonym
ACH, CD333, CEK2, HSFGFR3EX, JTK4

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 25
PP2 9,200
AG1478 22,000
D-64406 9,000
Scientific Information
P22607 (http://www.uniprot.org/uniprot/P22607)

Screening Location
Malvern, USA

Reference compound IC50 for FGFR3
Reference compound IC50 for FGFR3