FGFR4 Kinase Mutant Profiler Service

Activity-based FGFR4 mutant kinase panel for compound profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#

FGFR4

Enzyme

Human FGFR4

HGNC

FGFR4

Synonym

Fibroblast growth factor receptor 4 [Precursor], FGFR-4, JTK2, TKF

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s (nM)
Mutant Staurosporine PP2 AG1478 PDGFR I-III EGFR/ErbB Inhibitor D-64406
WT 110 2,400 >20,000 n.a. n.a. >20,000
N535K 4,800 22,000 27,000 n.a. >100,000 >100,000
V550E 3,700 >100,000 >100,000 n.a. >100,000 >100,000
V550L 70 >100,000 >100,000 >100,000 n.a. >100,000
V550M 28 >100,000 >100,000 70,000 n.a. 95,000
Screening Location

Malvern, USA