FGFR4 (V550M) Kinase Assay Service

Activity-based FGFR4 (V550M) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FGFR4 (V550M)
Enzyme
Human FGFR4 (V550M)
HGNC
FGFR4 (V550M)
Synonym
TKF, JTK2, MGC20292
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine28
PP2>100,000
AG1478>100,000
PDGFR I-III70,000
D-6440695,000
Scientific Information
P22455 (http://www.uniprot.org/uniprot/P22455)
Screening Location
Malvern, USA
Reference compound IC50 for FGFR4 (V550M)
Reference compound IC50 for FGFR4 (V550M)