Activity-based FGR kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
FGR
Enzyme
Human FGR
HGNC
FGR
Synonym
SRC2, c-fgr, p55c-fgr
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.9 |
| PP2 | 25 |
| AG1478 | 840 |
| D-64406 | 330 |
Scientific Information
P09769 (http://www.uniprot.org/uniprot/P09769)
Screening Location
Malvern, USA
Reference compound IC50 for FGR
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