FLT1 NanoBRET Kinase Assay

FLT1 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target

FLT1

HGNC Symbol

FLT1

Synonyms

FLT, VEGFR1, FLT1, VEGF-R1

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Format

384-well plate format using Promega’s NanoBRETTM TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-FLT1 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-9 and then treated with reference compound Foretinib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
Foretinib 6.969e-010
Screening Location

Malvern, USA

Reference Compound IC50 for FLT1