FLT1/VEGFR1 Kinase Assay Service

Activity-based FLT1/VEGFR1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FLT1

Enzyme
Human FLT1

HGNC
FLT1

Synonym
FLT, VEGFR1

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 11
PP2 3,900
AG1478 4,000
D-64406 6,400
Scientific Information
P17948 (http://www.uniprot.org/uniprot/P17948)

Screening Location
Malvern, USA

Reference compound IC50 for FLT1/VEGFR1
Reference compound IC50 for FLT1/VEGFR1

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