FLK2; STK1; CD135
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRETTM TE Intracellular Kinase Assay
HEK293 cells transiently expressing NanoLuc®- FLT3 (D835Y) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRETTM Tracer K-10 and then treated with reference compound Foretinib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.
Binding of compound to target kinase
Foretinib (IC50 value): 4.373e-009 M